2、实验与探究能力
现代生物科技专题
知识内容
要求
6-1 基因工程
（1）基因工程的诞生
（2）基因工程的原理及技术
（3）基因工程的应用
（4）蛋白质工程
Ⅰ
Ⅱ
6-2 克隆技术
（1）植物的组织培养
（2）动物的细胞培养与体细胞克隆
（3）细胞融合与单克隆抗体
6-3 胚胎工程
（1）动物胚胎发育的基本过程与胚胎工程的理论基础
（2）胚胎干细胞的移植
（3）胚胎工程的应用
6-4生物技术的安全性和伦理问题
（1）转基因生物的安全性
（2）生物武器对人类的威胁
（3）生物技术中的伦理问题
6-5 生态工程
（1）简述生态工程的原理
（2）生态工程的实例
7-1 基因工程
DNA的粗提取与鉴定
掌握程度参考本考试大纲中的：
一、考试的能力要求
5-3 生物技术在食品加工及其他方面的应用
（1）从生物材料中提取某些特定的成分
（2）运用发酵加工食品的基本方法
（3）测定食品加工中可能产生的有害物质
（4）植物的组织培养
（5）蛋白质的提取和分离
（6）PCR技术的基本操作和应用
5-2 酶的应用
（1）酶的存在与简单制作方法
（2）酶活力测定的一般原理和方法
（3）酶在食品制造和洗涤等方面的应用
（4）制备和应用固相化酶
2.实验与探究能力
生物知识内容表（二）
选考部分
生物技术实践
5-1 微生物的利用
（1）微生物的分离和培养
（2）某种微生物数量的测定
（3）培养基对微生物的选择利用
（4）利用微生物发酵来生产特定的产物以及微生物在其他方面的应用
4-3 稳态与环境
（1）探究植物生长调节剂对扦插枝条生根的作用
（2）模拟尿糖的检测
（3）探究培养液中酵母菌数量的动态变化
（4）土壤中动物类群丰富度的研究
（5）探究水族箱（鱼缸）中群落的演替
四、考核目标与要求
4-2 遗传与进化
（1）观察细胞的减数分裂
（2）低温诱导染色体加倍
（3）调查常见的人类遗传病
三、考试的能力要求
实 验
4-1 分子与细胞
（1）观察DNA、RNA在细胞中的分布
（2）检测生物组织中还原糖、脂肪、和蛋白质
（3）用显微镜观察多种多样的细胞
（4）观察线粒体和叶绿体
（5）通过模拟实验探究膜的透性
（6）观察植物细胞的质壁壁分离和复原
（7）探究影响酶活性的因素
（8）叶绿体色素的提取和分离
（9）探究酵母菌的呼吸方式
（10）观察细胞的有丝分裂
（11）模拟探究细胞表面积与体积的关系
二、考试的能力要求
M：Listen, I can see that you are in love with that plate．Let’s just make it $45．I’d really like you to take that home with you．
W：How about $35? I really can’t go any higher than that．
M：I really can’t, I mean that would be selling at cost．
W：Well, $38 is absolutely the most I can spend．
M：All right．I really shouldn’t．
Text 11
M：
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So come on down to Frontier Furniture．Located downtown two blocks east of city hall, across from Union Square．We’re open daily from 10:00 AM to 9:30 PM．So, come on in, and let us make your dream home a reality．
M：$50．
W：$50! That’s quite dear．I can’t afford that．
M：Remember these are hand painted pieces of pottery; that kind of work doesn’t come cheaply．
W：Yes, I understand that．But I really think that $50 is just too much．
M：Well, I have to leave home at twenty to seven, so I can catch a bus at 7:00．
W：And what time to you get to work?
M：It takes me about an hour to get there, but it stops right in front of my office．
W：That’s nice．And what time do you get off work?
M：Uh, around 5:00 o’clock．Then, we eat dinner around 6:30, and my wife and I Play with the kids until 8:00 or so．
W：So, when do you work on your website?
M：Well, my wife and I often watch TV until 10:00 o’clock．Then I work on my site, and sometimes stay up until the early hours of the morning．
Text 8
W：Sir, you’ve been using the Online Catalogue for quite a while; is there anything I can help you find?
M：I’ve got to write a paper about Hollywood in the 30s and 40s, and I’m really struggling．There are hundreds of books, and I just don’t know where to begin．
W：Your topic sounds pretty big．Why don’t you narrow it down to something like…uh…the history of the studios during that time ?
M：You know．I was thinking about doing that, but more than 30 books came up when I typed in “movie studios．”
W：You could cut that down even further by listing the specific years you want．Try adding “1930s” or “1940s” or maybe “Golden Age．”
M：”Golden Age” is a good idea．Let me type that in…Hey, look, just 6 books came up that time．That’s a lot better．
W：Oh…another thing you might consider…have you tried looking for any magazine or newspaper articles?
M：No, I’ve only been searching for books．
Text 9
W：Next．Uh, your passport please．
M：Okay．
W：Uh, what is the purpose of your visit?
M：I’m here to attend an important meeting on education for the first part of my trip, and then I plan on touring the capital for two or three days．
W：And where will you be staying?
W：I’ll be staying at a hotel downtown for the entire week．
M：And uh, what do you have in your luggage?
W：Uh, well, just, just my personal belongings．
M：Okay, Uh, please open your bag.
W：Sure．
M：Okay…Everything’s fine．Well, enjoy your trip．
W：Thanks．
Text 10
M：Hello, madam, what can I do for you today?
W：This is quite lovely．Where was it made?
M：Oh, I see you have excellent taste．Yes, that is beautiful, isn’t it? It’s the local pottery．
W：It’s really something…What about the price?