第一节   听力理解(5段共15小题;每小题2分,满分30分)

  每段播放两遍。各段后有几个小题,各段播放前每小题有5秒钟的阅题时间。请根据各段播放内容及其相关小题,在5秒钟内从题中所给的A、B、C三个选项中,选出最佳选项,并在答题卡上将该项涂黑。

听第一段对话,回答第1-3题

1. What are they mainly talking about?

  A. Going out for food.   B. Going shopping.    C. Going for a picnic.   

2. When will they go?

  A. This Sunday.      B. Next Saturday.     C. Next Sunday.

3. What will the man most probably do the day before they go?

  A. Prepare the car.    B. Buy some food.    C. Write the report.

听第二段对话,回答第4-6题

4. Why is Simon at home?

  A. He has a serious cold.  B. He hurt his leg.   C. He doesn’t want to climb the mountain.

5. Where does Janet most probably speak to Simon?

  A. At Simon’s home.    B. At Simon’s school.  C. In the hospital.

6. What will Simon do next?

  A. Go to see the doctor.   B. Call his teacher.    C. Take his medicine.

听第三段对话,回答第7-9题

7. What language may not be learned by the man?

  A. Chinese       B. Spanish        C. Japanese

8. What language is the man learning?

  A. Chinese       B. English        C. French

9. What does the woman think of language learning?

  A. In language learning only a few languages are difficult to learn.

  B. The language similar to one’s native language is easier to learn.

  C. The sound system is the hardest part of a language.

听第四段对话,回答第10-12题

10. What is the possible relationship between the two speakers?

   A. Colleagues.           B. Schoolmates.     C. Landlady and tenant (佃户).

11. How much does the man need to pay for one month?

   A. 1,200 dollars.                   B. 700 dollars.       C. 500 dollars.    

12. What does the man think of his room in the apartment?

   A. It’s very bright.    B. It’s quite big.      C. It’s comfortable.

听第五段独白,回答第13-15题

13. What does the speaker most probably do?

  A. A book seller.     B. A camera seller.      C. A photography teacher.

14. How can people take a great picture according to the speaker?

  A. They must obey some rules of photography.

  B. They must try new ways of photography.

  C. They must use the digital cameras to take pictures.

15. Which of the following is mainly talked about in the book?

  A. All good pictures come from digital cameras.

  B. There are some best ways to make a wonderful picture.

  C. How to improve the photography skill.

29.(16分)酶解法制备原生质体的原理是利用酶溶液对细胞壁成分的降解作用。蜗牛酶液从蜗牛(以植物为食)消化腺中提取;果胶酶、纤维素酶从微生物中提取。为了研究不同酶液的酶解效果,某实验小组取无菌烟草幼叶,切成相同大小的小片,等量放入6支试管中,试剂用量和实验结果列于下表。请回答有关问题。

    试管编号
项目
  I
  Ⅱ
  Ⅲ
   Ⅳ
   V
   Ⅵ
  蒸馏水(mL)
  2
   O
   1
   1
   1
   1
  缓冲液(mL)
  0
   2
   1
  0.5 
  0.5
  0.5
果胶酶液(mL)
  O
   0
   O
  0.5
   O
   0
蜗牛酶液(mL)
  O
   0
   O
   O
  0.5
   0
纤维素酶液(mL)
  0
   O
   0
   O
   0
  0.5
   实验结果
(绿色深浅程度)
-
-
 -
 +++
++++
++

(注:“+”越多表示绿色越深,“一”表示颜色无显著变化)

 (1)实验过程中,需要轻摇试管,其目的是    ,使原生质体从叶小片中游离出来,以便观察悬浮液绿色的深浅。

(2)从绿色的深浅可推测:蜗牛酶液酶解效果最好,原因是蜗牛酶液含有     等多种酶。该实验中    是空白对照组,其设置意义是    。

(3)用网筛过滤原生质体到离心管内,离心后收集沉淀物,并用     洗涤。

(4)原生质体是否符合要求还需进行检验,其检验的方法是     。

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