第一节 单项填空 (共15小题，每小题1分， 满分15分)
21. The lady mentioned a lot of ______ things I had experienced as if she had been _______student I once taught.
A. / ；a B. / ；the C. the ；a D. the ；the
22. We believe _________ you have devoted yourself to is sure to come true.
A. if B. who C. that D. what
23. I know you have placed great hope on me, but I’m afraid I might _______.
A. let you down B. let you out C. let you up D. let you alone
24. --- Ten dollars, please!
--- How terrible! I ______ to bring my wallet with me.
A. was forgetting B. forgot C. had forgotten D. am forgetting
25. It is your husband who ________ for what happened.
A. is to blame B. should blame C. is to be blamed D. could blame
26. He said he learned a little of Italian by talking steadily with the man ______ next to him on a flight from New York to Rome.
A. be seated B. seated C. to seat D. seating
27. --- What’s the matter with you?
--- Oh, I’m not feeling well in the stomach. I _____ so much fried fish just now.
A. shouldn’t eat B. mustn’t have eaten
C. shouldn’t have eaten D. mustn’t eat
28. Under no circumstances ___________ give up.
A. would we B. we wouldn’t C. we would D. wouldn’t we
29. The old man spoke to the girl again, raising his voice a bit, but still _______ no answer.
A. received B. receiving C. receive D. to receive
30. ------- What about going out?
------- I’ve got a headache. And _____, Jack’s coming to see me. I ought to stay in.
A. however B. anyhow C. though D. while
31. The beautiful village, ________ we spent our holiday in last month, lies at the foot of a mountain.
A. where B. that C. which D. what
32. --- Do you feel like _______ there or shall we take a bus?
--- I’d like to walk, but since there isn’t much time left, I’d rather we ______ a taxi.
A. walking; hire B. to walk; hire
C. to walk; hired D. walking; hired
33. ________ you had your photos taken with the foreigners?
A. It was where that B. Where it was that
C. Where was it that D. Where that was it
34. I _______ Jack to ring up to see if Mary was there.
A. suggested B. hoped C. advised D. made
35. --- What was the trouble with you the day before yesterday?
--- When I practiced running on the playground, my strength ________ and I fell onto the ground.
A. gave away B. gave in C. gave off D. gave out
29.下列三图分别表示基因的结构示意图和利用基因工程培育抗虫棉过程示意图。请据图回答下列有关问题：
(1)图C中的目的基因是从图A和图B中的哪一个图所表示的基因提取的?___________。图C中科学家在进行①操作时，通常要用______________分别切割运载体和含目的基因的DNA片段。
(2)图C中Ⅲ是导入目的基因的受体细胞，经培养、筛选获得一株有抗虫特性的转基因植株。经分析，该植株细胞的细胞核中含有一个携带目的基因的DNA片段，因此可以把它看作是杂合子。理论上，在该转基因植株自交产生的F1代中，仍具有抗虫特性的植株占总数的_____________。
(3)为了防止转入的抗虫性状随花粉散播到其他杂草中，应该将重组质粒转入受体细胞的______________中。以上述抗虫棉植株提供花粉与普通棉花杂交，F1代是否具有抗虫特性__________。
(4)若抗虫基因转入棉花后未表达，则需修饰该基因的 。
27.下图是植物组织培养的简略表示。请据此回答：
(1)②表示__________，这些细胞的新陈代谢类型是____________________。 (2)若想制作人工种子,应该选用(填编号) __________。 (3)若①是花药,则④是____________________。若要获得脱毒苗则要用____________区的细胞进行离题培养。 (4)若①是胡萝卜的橙黄色根细胞,则培养的④的叶片的颜色是_________,这说明根细胞含有 ____ __。
28(1)某同学在做微生物实验时，要在土壤中分别分离出圆褐固氮菌、金黄色葡萄球菌、硝化细菌和酵母菌混在一起，请你设计一个实验。
①配置pH值为5左右的培养基，可以分离出 ，还可以通过在培养基中加入 来选择该菌。
②要选择金黄色葡萄球菌，培养基中要加入 。
(2)用谷氨酸棒状杆菌发酵谷氨酸时，若产物为乳酸，但通入的无菌空气量正常，则可能的原因是 出现问题。谷氨酸是谷氨酸棒状杆菌的 代谢产物，若积累过量会引起 的调节。
26.将小鼠胚胎干细胞定向诱导分化成一种特定的细胞(命名为M细胞)，再将M细胞移植到糖尿病模型小鼠(胰岛细胞被特定药物破坏的小鼠)体内，然后小鼠的血糖浓度，结果如图所示(虚线表示正常小鼠的血糖浓度值)。请回答相关问题：
(1)实验用的胚胎干细胞取自小鼠的早期囊胚，取出胚胎后一般用 酶将其分散成单个细胞。
(2)根据实验结果可以判定M细胞已具有 细胞的功能。
(3)移植后，小鼠血糖降低这是因为胰岛素具有
的作用。
(4)当葡萄糖在细胞中进行无氧呼吸，其产物进入血液可与缓冲物质中的 (写出具体的化合物)发生反应。
(5)用胰岛素基因片段做探针，对小鼠胚胎干细胞和M细胞进行检测。请在下表的空格中填上检测结果(用“+”表示能检测到，用“-”表示不能检测到)。
25.下列关于微生物的叙述，错误的是
A．调整期微生物适应环境，进行了酶合成的调节
B．组成酶只受基因的控制
C．酶活性的调节属于反馈调节
D．一种病毒合成一种蛋白质
24.下列关于动物细胞工程的叙述，正确的是
A．生物导弹是用癌细胞的单克隆抗体杀死癌细胞
B．动物细胞培养的基础是细胞具有全能型
C．细胞融合是利用了细胞膜的结构特点
D．制备单克隆抗体的第二次筛选是选出杂交瘤细胞
23.在光照下，供给玉米离体叶片少量的14CO2，随着光合作用时间的延续，在光合作用固定CO2形成的C3化合物和C4化合物中，14C含量变化示意图正确的是
下图是植物组织培养的简略表示。请据此回答：
