ÌâÄ¿ÄÚÈÝ

£¨2014Çï•ÖØÇìÆÚÄ©£©ÈçÌâͼ1ËùʾΪijÉú̬ϵͳÄÜÁ¿Á÷¶¯µÄ¶¨Á¿·ÖÎöͼ½â£¬Í¼ÖÐA¡¢B¡¢C´ú±íÈý¸öÓªÑø¼¶£¬Êý×Ö¾ùΪʵ¼Ê²âµÃµÄÄÜÁ¿ÊýÖµ£¬µ¥Î»ÎªJ/£¨cm2•a£©£»Ìâ8ͼ2ÊǸÃÉú̬ϵͳÖеÄÖ÷ÒªÖÖȺ¹¹³ÉµÄʳÎï¹ØÏµÍø£®Çë·ÖÎö»Ø´ð£º

£¨1£©Í¼1±íÃ÷£¬¸ÃÉú̬ϵͳµÄÉú²úÕ߹̶¨µÄÌ«ÑôÄÜ×ÜÁ¿ÊÇ J/£¨cm2•a£©£®Èç¹û¸ÃÉú̬ϵͳΪɭÁÖÉú̬ϵͳ£¬½«É­ÁÖÈ«²¿¿³·¥£¬Ôò´ÓÈ«²¿¿³·¥µÄµØ·½¿ªÊ¼µÄÑÝÌæÎª £®

£¨2£©Í¼1ÖÐ £¨Ìî×Öĸ£©ºÍ·Ö½âÕßÔÚ̼ѭ»·ÖÐÆð׏ؼü×÷Óã¬ÄÜÁ¿ÔÚµÚÒ»ÓªÑø¼¶ºÍµÚ¶þÓªÑø¼¶Ö®¼äµÄ´«µÝЧÂÊΪ £¨±£ÁôÕûÊý£©£®

£¨3£©Í¼1ÖУ¬Èç¹ûAÖ÷ҪΪÀ¶Ô壬ÈçÒòË®Ìå¸»ÓªÑø»¯¶ø³öÏÖË®»ª£¬¿ÉÒÔ˵Ã÷¾ßÓÐÉú̬ϵͳÓÐ £¨ÎïÖÊÑ­»·/ÄÜÁ¿Á÷¶¯£©¹¦ÄÜ£®

£¨4£©Í¼2ÖУ¬´¦ÓÚͼ1ÖÐBÓªÑø¼¶µÄÖÖȺÓÐ £¬ÒҺͱûµÄÖÖ¼ä¹ØÏµÎª £®Ì¼ÔªËØÔڼס«ÎìÖ®¼äµÄÖ÷Òª´«µÝÐÎʽÊÇ £®

Á·Ï°²áϵÁдð°¸
Ïà¹ØÌâÄ¿
1£®¿ÆÑÐÈËԱͨ¹ý»ùÒò¹¤³ÌʵÏÖÁ˦Â-¸Ê¶¾ÛÌÇø»ùÒòÔÚÖí³¦µÀÒ°Éú½Íĸ¾úÖеıí´ï£¬Ê¹Ô­À´²»Äܱ»ÖíÀûÓõĴÖÏËάÔÚÖí³¦µÀÄÚ±»·Ö½â³É¿ÉÖ±½ÓÎüÊյĵ¥ÌÇ£®Í¼Îª¹¹½¨¹¤³Ì¾úµÄ²¿·Ö¹ý³Ì£¬ÆäÖн鵼ÐòÁÐÄÜÒýµ¼ÔØÌåÕûºÏµ½½Íĸ¾úµÄȾɫÌåDNAÉÏ£®Çë½áºÏÏà¹ØÖªÊ¶£¬»Ø´ðÏÂÁÐÎÊÌ⣺

£¨1£©ÀûÓÃPCR¼¼Êõ´ÓÒ°Éú½Íĸ¾úȾɫÌåDNAÖлñȡĿµÄ»ùÒòµÄǰÌáÊÇÀûÓÃÒ»¶ÎÒÑ֪ĿµÄ»ùÒòµÄºËÜÕËáÐòÁкϳÉÒýÎ
£¨2£©Èç±íΪËÄÖÖÏÞÖÆÃ¸µÄʶ±ðÐòÁкÍÇиîλµã£¬ÓñíÖÐËÄÖÖÏÞÖÆÃ¸ÇиîDNA£¬Äܵõ½ÏàͬµÄð¤ÐÔÄ©¶ËµÄÊÇÏÞÖÆÃ¸BamH¢ñºÍBgl¢ò£®
ÏÞÖÆÃ¸Ê¶±ðÐòÁкÍÇиîλµã
EcoR I¡ý
GAATTC
BamH I¡ý
GGATCC
Bgl¢ò¡ý
AGATCT
Sal I¡ý
GTCGAC
£¨3£©ÔÚ¹¹½¨½éµ¼ÐòÁÐÖÊÁ£ÔØÌåʱ£¬Ñ¡ÓÃEcoR IºÍBamH IͬʱÇиîÖÊÁ£ºÍ½éµ¼ÐòÁеÄÄ¿µÄÊDZÜÃâÖÊÁ£ºÍ½éµ¼ÐòÁз¢Éú×ÔÉí»·»¯£®
£¨4£©ÔÚ¹¹½¨ÖØ×é±í´ïÔØÌåʱ£¬ÒýÈëÆô¶¯×ÓµÄÄ¿µÄÊÇÇý¶¯Ä¿µÄ»ùÒòת¼³ömRNA£®
£¨5£©¶ÔÖØ×é±í´ïÔØÌå½øÐÐøÇмø¶¨£¬¼ÙÉèËùÓõÄø¾ù¿É½«Ê¶±ðλµãÍêÈ«Çпª£®ÈôʹÓÃEcoR IºÍSal IøÇУ¬½«µÃµ½3ÖÖDNAƬ¶Ï£®

Î¥·¨ºÍ²»Á¼ÐÅÏ¢¾Ù±¨µç»°£º027-86699610 ¾Ù±¨ÓÊÏ䣺58377363@163.com

¾«Ó¢¼Ò½ÌÍø