23.---Do you feel like _____ there or shall we take a bus?

---I’d like to walk,but since there isn’t much time left,I’d rather we_____ a taxi.

A.walking; hire　　　 B. to walk; hire　　　 C. to walk; hired　　 D. walking; hired

22.Mary wrote an article on _____ the team had failed to win the game.

A.why　 　　　　　　　　　　　　 B. what 　　　　　　　　　　　 C. who 　　　　　　　　　　　　 D. that

21.Follow your doctor’s advice,_____ your cough will get worse.

A.or　 　　　　　　　　　　　　　 B. and 　　　　　　　　　　　　 C. then 　　　　　　　　　　　　 D. so

20.This is the small house less than 15 square meters,under _____ roof lives a large family of three generations.

A. which　 　　　　　　　　　　 B. that　 　　　　　　　　　　　 C. its　 　　　　　　　　　　　　　 D. whose

19._____ is reported in the newspaper is that seventeen climbers have been killed in the snowstorm.

A.It　 　　　　　　　　　　　　　 B. As　 　　　　　　　　　　　　　 C.What 　　　　　　　　　　　　 D.That

18.The little girl insisted that what she said ________ true.

A．was　　　　　　　　　　　　 B．is　　　　　　　　　　　 C．should be　　　　　 D．were

17. They can't be working now. It's time they ________ the office.

A．have left　　　　　　　　　　　 B．would leave　　　 C．could leave　　　 D．left

第一节 语法和词汇知识(共15小题；每小题1分，满分15分)

16._____ ,she is the sort of woman to spread sunshine to people through her smile.

A.Shy and cautious　 　　　　　　　　　　　　　　　　　 B.Sensitive and thoughtful

C.Honest and confident 　　　　　　　　　　　　　　　 D.Light-hearted and optimistic

听第6段材料，回答第6至7题。

1.　　　 (6) When will the woman show the man the apartment?

A．　　　　　　　 Next Thursday.

B． Right now.

C． This afternoon.

2.　　　 (7) What is the apartment like?

A. 　It’s lovely and very big.　　　

B. 　It’s a good house with a big kitchen .

C. 　It’s a quiet place with a sunny window.

听第7段材料，回答第8至9题。

3.　　　 (8) How many rolls of film did the man develop last year?

A．　　　　　　　 About 6.

B． About 5.

C． About 3.

4.　　　 (9) What does the man like to take pictures of?

A. 　Anything that attracts his attention.　　　　　　　　　　

B. 　Anything that shows true feelings.　　　　　　　　　　　　　　　

C. 　Anything that makes people happy.

听第8段材料，回答第10至12题。

5. (10) What is the probable relationship between the two speakers?

A. 　Interviewer and interviewee

B. 　Father and daughter.

C. 　Friends.

6.(11) What has the woman done recently?

A. Built a new building for her father.　　　　　 　

B. Helped her father design some buildings.

C. Learned some design skills from her father.

7(12) Why does the woman want to work in the man’s company?

A．　　　　　　　 She wants to be trusted.　　　　　　　　

B． She want to design buildings.　　　　　　　　　　　

C． She wants to work with her friend.

听第9段材料，回答第13至16题。

8(13) What will the man do for summer break?

A.　　　 Go traveling.

B.　　　 Go back home.　　　　　　　　　　　　　　　　

C.　　　 Go to visit his friends.

9(14)Where is the woman going for her trip?

A. Her hometown.　　　 B.Mary’s home.　　　　　　　　 C. Suburban areas.

10.(15) How will the woman travel?

A.By bike.　　　　　　 　　　 B. By air.　　　　　　　　　　　　　　 C. By bus.

11.(16) How long will the speakers’ summer break last?

A. Seven days.

B. A couple of weeks.

C. Two months.

听第10段材料，回答第17至20题。

12.(17) Why wasn’t the speaker afraid of the cries of strange animals?

A. He had never been to the forest before.　　　　

B. He thought they were interesting.　　　　　　　　　

C. He thought they were natural .

13.(18) What attracted the speaker’s father’s interest most?

A. The strange animals.　　　　　　　　　　　　　

B. The special plants.　　　　　　　　　　　　　　　

C. The singing birds.

14.(19) What did the tiger intend to do?

A.Drink some water.　 B.Struggle with the crocodile.　 C.Hunt for food.　　 　　　　　　

15(20) What filled the speaker and his father with terror?

A. The crocodile’s sudden appearance.

B.The tiger’s falling into the water.

C.The tiger’s howl of pain and anger.

55、图为某种质粒表到载体简图，小箭头所指分别为限制性内切酶EcoRI、BamHI的酶切位点，　 amp^R为青霉素抗性基因，tct^R 为四环素抗性基因，P启动子，T为终止子，ori为复制原点。已知目的基因的两端分别有包括EcoRI、BamHI在内的多种酶的酶切位点。

据图回答：

(1)将含有目的基因的DNA与质粒该表达载体分别用EcoRI酶切，酶切产物用DNA连接酶进行连接后，其中由两个DNA片段之间连接形成的产物有　　　 、　　　　 、　　

　　　 三种 。若要从这些连接产物中分离出重组质粒，需要对这些连接产物进行　　　　 。

(2)用上述3种连接产物与无任何抗药性的原核宿主细胞进行转化实验。之后将这些宿主细胞接种到含四环素的培养基中，能生长的原核宿主细胞所含有的连接产物是　　　　 ；

(3)在上述实验中，为了防止目的基因和基因和质粒表达载体在酶切后产生的末端发生任意连接，酶切时应选用的酶是　　　　　　　　 。

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