题目内容

【题目】利用细胞工程方法，以SARS病毒核衣壳蛋白为抗原制备出单克隆抗体。下列相关叙述正确的是（ ）

A．用纯化的核衣壳蛋白反复注射到小鼠体内，产生的血清抗体为单克隆抗体

B．体外培养单个效应B细胞可以获得大量针对SARS病毒的单克隆抗体

C．将等量效应B细胞和骨髓瘤细胞混合，经PEG诱导融合后的细胞均为杂交瘤细胞

D．利用该单克隆抗体与SARS病毒核衣壳蛋白特异性结合的方法可诊断出病毒感染者

【答案】D

【解析】单克隆抗体是效应B细胞与骨髓瘤细胞融合形成的杂交瘤细胞分泌的，A错误；单独的效应B细胞能产生抗体，但不能无限增殖，因此在体外培养的情况下，不能得到大量的单克隆抗体，B错误；将等量效应B细胞和骨髓瘤细胞混合，经PEG诱导融合后的细胞有效应B细胞自身融合细胞、骨髓瘤细胞自身融合细胞和杂交瘤细胞三种，C错误；单克隆抗体具有特异性强、灵敏度高的特点，因此利用该单克隆抗体与SARS病毒核衣壳蛋白特异性结合的方法可诊断出病毒感染者，D正确。

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【题目】书面表达

假设你是李华，你市和美国某城市为友好城市，某国际交流中心将利用暑假组织一个美国中学生交流活动，并在网上征集可提供住宿的中国家庭。你认为自己的家庭符合要求，请你给活动负责人写一封申请信。内容如下：

1. 有一间独立卧室；

2. 家庭成员具备良好的素质；

3. 能够用英语交流。

注意：

1. 词数100左右（开头和结尾已给出，不计入总词数）；

2. 可适当增加细节，以使行文连贯。

Dear Sir or Madam,

____________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________

Yours,

Li Hua

【题目】七选五

根据短文内容，从短文后的七个选项中选出能填入空白处的最佳选项。选项中有两项为多余选项。

Happy birthday! Do birthdays really make people happy? 1 Birthdays celebrate the day when we were born. Besides, that extra candle on the cake suggests another year of growth and maturity(成熟) — or so we hope. We all like to imagine that we are getting wiser and not just older. Most of us enjoy seeing the wonder of growth in others as well. For instance, seeing our children develop and learn new things makes us feel proud. For Americans, like people in most cultures, growing up is a wonderful process. But growing old? 2

Growing old is not exactly for people in youth-oriented American culture. 3 As the old saying goes, ＂You’re young as you feel.＂ Older people joke about how many years young they are, rather than how many years old. People in some countries value the aged as a source of experience and wisdom. But Americans seem to favor those that are young, or at least ＂young at heart＂.

Many older Americans find the ＂golden years＂ to be anything but golden. Economically, ＂senior citizens＂ often struggle just to get by. Retirement at the age of 65 brings a sharp decrease in personal income. Social security benefits usually cannot make up the difference. Older people may suffer from poor nutrition, medical care, and housing. 4 American sociologist Pat Moore once dressed up like an older person and wandered city streets. She was often treated rudely — even cheated and robbed. However, dressed as a young person, she received much more respect.

5 Why? People are living longer. Fewer babies are being born. And middle-aged ＂baby boomers＂ are rapidly entering the group of the elderly. America may soon be a place where wrinkles(皱纹) are ＂in＂. Marketing experts are already noticing this growing group of consumers.

A. Of course they do.

B. That is a different story.

C. Most people in America like the young.

D. Some even experience age discrimination(歧视).

E. The population in America grows larger every year.

F. Most Americans like to look young, act young and feel young.

G. Unfortunately, the elderly population in America is increasing fast.

【题目】A、B、C、D、E五种元素的原子序数依次递增，且均属于短周期元素，已知A是非金属元素，其电子层数与最外层电子数相等。Bn＋和C（n＋1）＋与氖原子具有相同的电子层结构，且C是两性元素。Dn－、E（n－1）－与氩原子具有相同的电子层结构。

（1）写出这五种元素的元素符号

A．__________；B.__________；C.__________；D.__________；E.__________。

（2）写出E单质与NaOH溶液反应的化学方程式：____________________。

（3）比较A、B、C、D、E的半径大小_____________________。

【题目】单词拼写

结合语境，根据汉语提示用单词的适当形式填空。

1. The salesman ________(说服) us to buy his product.

2. She is a ________(毕业生) student in literature.

3. They________(终于) realized that the whole thing was a joke.

4. They have planned a tight________(日程表) of travel.

5. He has the ability to________(组织).

6. The workers are ________(决定) to finish the job on time.

7. It is one day’s________(旅程) to get to the seaside.

8. There were perhaps ten________(步) between me and the bear.

【题目】原子序数为11～17号的元素，随核电荷数的递增，以下各项内容的变化是［填“增大（强）”、“减小（弱）”或“相同（不变）”］。

（1）各元素的原子半径依次_________，其原因是____________。

（2）各元素原子的电子层数__________，最外层电子数依次_____________。

（3）元素的金属性逐渐__________ ，而非金属性逐渐__________，元素失电子能力逐渐__________ ，得电子能力逐渐__________ 。

【题目】 完形填空（每小题1分，满分20分）

阅读下面短文，从每题所给的A、B、C和D四个选项中，选出最佳选项。

Last week I was in the boarding area of the airport excited about my Easter trip home to see my parents. I was busy planning for the weekend, 1 I was taken out of my daydreaming by an elderly woman talking __2__ into her cellphone. She kept telling the person to 3 her where she was. She 4 the gate number again and again. She got a little louder each time she did that. Many people snickered (窃笑) because she was nearly __5__ and thought how silly old people could be.

Then I heard her say, ＂I’m 6 , but I can’t hear you. There seems to be something wrong with my hearing aid.＂ ＂That’s too 7 .＂ I thought as I moved forward to board the flight. Then I noticed her __8__ was becoming increasingly frantic (发狂似的) as she tried to understand the person on the other end of the phone. It was almost my 9 to board. I couldn’t 10 looking back at the woman. It made me think how __11__ I was to have my hearing. And I realized I could offer her some 12 .

I suddenly got out of the 13 and went to the woman. I showed her to 14 me the phone and shouted, ＂Let me help you. I’ll tell you what the person is 15 .＂ She looked at me and agreed 16 . I took the phone from her,17 who I was, listened for a moment, and told the woman her husband was __18__ her outside the airport as he could not enter the gate area. Finally, I pointed her in the right 19 .

It took me less than two minutes to finish the task, but my 20 in having been able to help her remains strong.

【1】A. but B. if C. so D. unless

【2】A. secretly B. loudly C. sadly D. quickly

【3】A. call B. help C. tell D. meet

【4】A. asked B. wrote C. repeated D. guessed

【5】A. shouting B. singing C. leaving D. dying

【6】A. sick B. tired C. sorry D. busy

【7】A. bad B. funny C. possible D. strange

【8】A. step B. tone C. friend D. mind

【9】A. duty B. idea C. task D. turn

【10】A. avoid B. stop C. continue D. keep

【11】A. normal B. lucky C. proud D. healthy

【12】A. money B. comfort C. help D. guide

【13】A. line B. space C. circle D. thought

【14】A. throw B. examine C. hand D. fix

【15】A. doing B. saying C. watching D. planning

【16】 A. honestly B. bravely C. angrily D. thankfully

【17】A. explained B. considered C. forgot D. lied

【18】A. thinking of B. turning to C. waiting for D. staring at

【19】A. queue B. direction C. street D. room

【20】A. pity B. hope C. love D. pride

【题目】为了进一步增强洗衣粉对血渍、奶渍、油污、土豆泥等衣物上常见污垢的去除能力，洗衣粉中常常会添加各种酶类。请回答有关问题：

（1）根据衣物上常见的污垢，加酶洗衣粉中添加的酶类有碱性蛋白酶、 、 和 等四类。

（2）碱性蛋白酶能将血渍、奶渍等含有的大分子蛋白质水解成可溶性的 。

（3）下图为加酶洗衣粉的包装袋上的产品说明，根据你所学习的有关知识，解释注意事项③的原因 。

注意事项

①请将本品置于阴凉、干燥处，避热、避湿、避光。

②不慎溅入眼中，立即用水冲洗。

③水温以30～50℃为宜，切忌用70℃以上的热水。

（4）下面是添加脂肪酶的加酶洗衣粉和普通洗衣粉洗涤效果的有关探究实验，请据表回答：

组别

洗涤物（等量）

洗涤温度

洗衣粉（等量）

水量

洗净所需时间

1

油污布

45℃

加酶

2L

4 min

2

油污布

45℃

普通

2L

7 min

3

油污布

5℃

加酶

2L

9 min

4

油污布

5℃

普通

2L

8 min

①该实验设计体现了 原则。

②若1号实验组洗净所需时间接近7分钟，请分析最可能的原因 。

（5）添加蛋白酶的加酶洗衣粉不适宜洗涤下列哪些衣料 。

①棉织品

②毛织品

③腈纶织品

④蚕丝织品

⑤涤纶织品

⑥锦纶织品

【题目】The old man insisted that I __________ his wallet and insisted that I __________ to the police station with him.

A. had taken; go B. take; should go

C. would take; had gone D. should take; go

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